Discover Glen Research's newest products along with a technical note on sugar conformations and modifications, product review of rhodamine dyes, and more in the latest Glen Report
MGB Eclipse® pairs a minor groove binding (MGB) tripeptide with a dark quencher. The MGB significantly enhances hybridization, while the Eclipse® has a broad absorption range that quenches many of the most common fluorophores, including FAM, HEX, TET and Yakima Yellow®.
Our line of fluorophore labels has expanded with the addition of new internal fluorophore-dT phosphoramidites, including JOE-dT, Quasar® 570-dT, and Quasar® 670-dT.
Since the DMT group binds strongly to reverse phase supports, the full-length sequences can be retained in a cartridge while the failure sequences are eluted.
The cycloaddition between azide- and alkyne-labeled entities is an excellent method to facilitate oligonucleotide conjugations. While alkyne labeling of oligonucleotides is more straightforward, there will always be situations where an azide is necessary. A facile method of obtaining azide-labeled oligonucleotides is to use 3’-Azido-Modifier Serinol CPG.
Explore Oligonucleotide Applications
Glen Research offers a wide range of products for oligonucleotide applications.
Glen Research offers a wide selection of fluorophores and fluorescence quenchers, which can be added during oligonucleotide synthesis at the 5′- or 3’-terminus or within the sequence. The resultant fluorescent oligonucleotide probes are typically used in a wide variety of applications like genetic and genome analyses, and forensic analysis. Common applications of fluorescent oligonucleotide probes are FRET and Molecular Beacon probes.