Design of Fluorescent Probes

Glen Research offers a wide selection of fluorophores and fluorescence quenchers, which can be added during oligonucleotide synthesis at the 5′- or 3’-terminus or within the sequence. The resultant fluorescent oligonucleotide probes are typically used in a wide variety of applications like genetic and genome analyses, and forensic analysis. Common applications of fluorescent oligonucleotide probes are FRET and Molecular Beacons probes. 

Glen Research products for the design of fluorescent probes are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by an Analytical Report and an HPLC trace, showing the results of our QC testing. Glen Research monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination.

Explore Design of Fluorescent Probes

With the growing popularity of red and near-infrared dyes, we are offering the Black Hole QuencherTM dyes (BHQs).

We are happy to offer several products containing the BlackBerry® Quencher (BBQ-650®), which exhibits a broad absorption profile from 550nm to 750nm, centered at 650nm.

Synthetic oligonucleotides with covalently-attached CDPI3 have enhanced DNA affinity and have improved the hybridization properties of sequence-specific DNA probes.

Cyclooctatetraene (COT) Serinol Phosphoramidite provides a means to improve the photostability of virtually any fluorophore in a modular fashion.

Two cyanine derivatives, Cyanine 3 and Cyanine 5, which differ in structure simply by the number of carbons in the conjugated poly-ene linkage, are joined by the closely related analogues, Cyanine 3.5 and Cyanine 5.5, and are available as phosphoramidites.

The Dabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus.

Glen Research’s agreement with ELITechGroup, formerly Epoch Biosciences, allows us to offer several of their proprietary products designed for the synthesis of novel DNA probes.

Dichloro-diphenyl-fluorescein, SIMA (HEX) exhibits virtually identical absorbance and emission spectra to HEX.

Many different fluorophores can be readily attached to oligonucleotides to create fluorescent probes, which form the basis of the detection systems used in DNA sequencing, forensic science and genetic analysis.

QUAL probes consist of two oligonucleotides, the first containing a nucleophilic group at the 3’-terminus, while the second has an electrophilic group at the 5’-terminus.

We offer 3’-TAMRA CPG for 3’ labeling and TAMRA-dT for labeling within the sequence. We also offer TAMRA NHS ester for labeling amino-modified oligonucleotides.