Glen Research offers a wide selection of fluorophores and fluorescence quenchers, which can be added during oligonucleotide synthesis at the 5′- or 3’-terminus or within the sequence. The resultant fluorescent oligonucleotide probes are typically used in a wide variety of applications like genetic and genome analyses, and forensic analysis. Common applications of fluorescent oligonucleotide probes are FRET and Molecular Beacons probes.
Glen Research products for the design of fluorescent probes are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by an Analytical Report and an HPLC trace, showing the results of our QC testing. Glen Research monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination.
An NHS ester allows the functionalization of an amino moiety in a variety of molecules, including DNA and RNA oligonucleotides, as well as peptides or proteins. NHS esters are especially useful in situations where the equivalent phosphoramidites exhibit instability to the conditions of oligonucleotide synthesis and/or deprotection.