Glen Research products for backbone modification are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by an Analytical Report and an HPLC trace, showing the results of our QC testing. Glen Research monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination.
Oligonucleotide synthesis is routinely carried out from the 3' to the 5' terminus for no other reason than the ease of synthesis of the monomer units. Glen Research offers phosphoramidites and supports for backwards oligonucleotide synthesis.
Methyl Phosphonamidites may be used in DNA synthesizers following conventional CE Phosphoramidite protocols to produce oligonucleotides containing one or more methyl phosphonate linkages.
Glen Research supplies methyl phosphoramidites in addition to β-cyanoethyl (CE) phosphoramidites for the few situations where the more labile cyanoethyl group is not an advantage.
Oligonucleotides containing LNA exhibit unprecedented thermal stabilities towards complementary DNA and RNA, which allows excellent mismatch discrimination.
Beta L-DNA is the mirror image version of naturally occurring D-DNA. L-DNA and D-DNA share identical structures that differ only in terms of stereochemistry and generally have identical physical and chemical properties.
To avoid polymerase extension at the 3' terminus, 2',3'-dideoxynucleoside and 3'-deoxynucleoside CPGs have proved to be effective. 2',3'-
A unique feature of 2'-5' linked oligonucleotides is their ability to bind selectively to complementary RNA.