Poly-Pak™ Reverse Phase Purification Products

The use of Poly-Pak™ packings in cartridges or barrels overcomes several disadvantages usually associated with reverse phase (RP) cartridges.  The packing is stable in the pH range 1-13, thus the ammonium hydroxide solution, diluted with water, is loaded directly onto the packing.  Also, after elution of failure sequences, the trityl group is removed and washed from the support-bound oligonucleotide.  The fully deprotected product can then be eluted and isolated by lyophilization.  Poly-Pak™ Cartridges may also be used for desalting normal or labeled oligonucleotides.  The original Poly-Pak cartridge and barrel are designed for 0.2 µmole syntheses or less.  Poly-Pak II cartridges and barrels are designed for use with 1 µmole syntheses.  A booklet, User Guide To Poly-Pak™ Cartridge Purification, describes in detail the process and several applications.  This booklet is available online at:  http://www.glenresearch.com/Technical/PolyPakBooklet.pdf

 
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