Glen-Pak™ cartridges have advantages over Poly-Pak cartridges in that a single loading of the diluted crude deprotection solution is all that is necessary. Also, the range of purification has been extended to 100+ using DMT-on oligos. Glen-Pak cartridges have similar performance to Fluoro-Pak cartridges but without the need for the fluorous DMT group at the 5' terminus and special phosphoramidites, so the cost is lower. In addition, Glen-Pak cartridges allow purification of virtually the complete range of dyes and modifiers. The Glen-Pak Cartridge 3g is a large cartridge capable of purifying 10-20 µmole oligonucleotide syntheses using the standard DMT-on procedure and Glen-Pak 30mg 96-Well Plates are for parallel purification of up to 50 nmole scale syntheses. The Glen-Pak 3mg 384-Well Plate is designed for use with 384-well plate compatible vacuum manifold systems and can purify up to a 20 nmole scale synthesis. Each well contains 3mg of Glen-Pak resin, which binds about 15 nmoles of full length 40-mer DMT-ON oligo.
Scale suggestions for the Glen-Pak product line are shown below:
| Glen-Pak Product | Catalog Number | Synthesis Scale Compatibility | |
| Glen-Pak 50mg Purification Cartridge | 60-5000-96 | 0 nmole - 200 nmole | |
| Glen-Pak Purification Cartridge | 60-5100-XX and 60-5200-XX | 10 nmole - 1.0 µmole | |
| Glen-Pak Cartridge 3g | 60-5300-01 | 5 µmole - 20 µmole | |
| Glen-Pak 30mg 96-Well Plate | 60-5400-01 | 10 nmole - 50 nmole | |
| Glen-Pak 3mg 384-Well Plate | 60-5500-XX | Up to 20 nmoles |
A User Guide to Glen-Pak™ Purification describes in detail the process and several applications for DNA and RNA purification.
This booklet is available online at: https://www.glenresearch.com/media/folio3/productattachments/application_guides/glen-pak_2022_2.pdf.
