|Fluorophores are very useful and sensitive labels for biomolecules, and fluorescent labels are by far the most common labels used for synthetic oligonucleotides. Many different fluorophores can be readily attached to oligonucleotides to create fluorescent probes, which form the basis of the detection systems used in DNA sequencing, forensic science and genetic analysis. A further subset of fluorescent probes is quenched fluorescent probes that contain a quencher molecule and are non fluorescent until the target is detected.
Fluorescent labels are available as phosphoramidites to modify oligonucleotides at the 5' terminus or within the sequence, as well as being available on supports for 3' modification. Quenchers are also available as phosphoramidites for 5' and internal labeling and as supports for 3' labeling.
3-(5,6,4',7'-tetrachloro-5'-methyl-3',6'-dipivaloylfluorescein-2-yl)-propanamido-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG
3-(3-oxo-7-pivaloylphenoxazin-2-yl)-propanamido-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG
1-[3-(4-monomethoxytrityloxy)propyl]-3,3,3',3'-tetramethylindodicarbocyanine chloride-1'-propyl-3-O-succinoyl-long chain alkylamino-CPG
1-[3-(4-monomethoxytrityloxy)propyl]-3,3,3',3'-tetramethylindocarbocyanine chloride-1'-propyl-3-O-succinoyl-long chain alkylamino-CPG
1-Dimethoxytrityloxy-3-[O-(N-carboxy-(Tetramethyl-rhodamine)-3-aminopropyl)]-propyl-2-O-succinoyl-long chain alkylamino-CPG