Some of our more popular minor base and modifier supports are available on polystyrene in columns fully compatible with the Applied Biosystems 3900 synthesizer. These include our popular Universal Support III, which will allow DNA, RNA or LNA oligos to be produced on the 3900 with ANY base at the 3' terminus. At the same time, we are offering 1 μmole columns of Universal Support III for the 3900 instrument. Structures and more complete descriptions are found in the relevant catalog sections for each item. AB 3900 columns can be prepared with virtually any of the CPG supports in this catalog. It is no longer necessary to adjust the flow using our AB 3900 CPG columns, as noted in the box to the right. Modified CPG columns are only available in 200 nmole size - simple add ‘A’ to the regular catalog number to order.
The spectral characteristics of this dye is detailed here.
Details
Usage
Coupling: Use either dmf-dG (10-1029-xx) with standard monomers and Cap A or use UltraMILD monomers (dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx ) and UltraMILD Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: If standard monomers were used, deprotect in tert-butylamine/water 1:3 (v/v) for 6 hours at 60°C. If UltraMILD monomers were used, deprotect in 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature. See Technical Bulletin for details. for details. Note: A side reaction can occur that leads to a loss of the TAMRA label. Treatment of the support prior to cleavage/deprotection with a hindered base, such as 10% diethylamine in ACN, for 3-5 minutes will minimize the loss of label.
Specifications
Storage
Freezer storage, -10 to -30°C, dry. Light sensitive material.
