AquaPhluor® 639 is a rhodamine dye with an excitation and emission in the far red of the visible spectrum and is a logical alternative to Cyanine 5. Like Cyanine 5, AP639 is relatively bright and exhibits sequence- and pH-independent fluorescence. Unlike Cyanine 5, AP639 also shows excellent chemical and photochemical stability as well as temperature-independent fluorescence. AP639 is available as the phosphoramidite and CPG for 5’ and 3’ labeling, respectively.
Details
Usage
Coupling: 3 minute coupling time recommended
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent
Anhydrous Acetonitrile
Storage
Freezer storage, -10 to -30°C, dry
Stability
1-2 Days
Intellectual Property
Product is subject to proprietary rights of ELITechGroup and is made and sold under license from ELITechGroup. For additional information, please contact ELITechGroup. AquaPhluor® is a registered trademark of ELITechGroup.
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.