5'-AquaPhluor® 639 CE Phosphoramidite

6-[2,3,16,17-tetrahydro-5,5,7,11,13,13-hexamethyl-1H,5H,13H,15H- Xantheno[2,3,4-ij:5,6,7-i'j′]diquinolizin-18-ium-9-yl-[2-chloro-6- (N-hydroxysulfonylethyl-N’-methylaminocarbonyl)-phen-4-yl] [trifluoroacetylaminobutoxy]phosphoryl][oxy]-hexyl-1-O- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite

Product Specifications

Formula:
C62H82ClF3N6O11P2S
M.W.:
1273.82
F.W.:
968.43
To Retrieve a Catalog Number, Select a Pack Size and Format:

Description

AquaPhluor® 639 is a rhodamine dye with an excitation and emission in the far red of the visible spectrum and is a logical alternative to Cyanine 5. Like Cyanine 5, AP639 is relatively bright and exhibits sequence- and pH-independent fluorescence. Unlike Cyanine 5, AP639 also shows excellent chemical and photochemical stability as well as temperature-independent fluorescence. AP639 is available as the phosphoramidite and CPG for 5’ and 3’ labeling, respectively.

Details

Usage

  • Coupling: 3 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 1-2 Days

Intellectual Property

Product is subject to proprietary rights of ELITechGroup and is made and sold under license from ELITechGroup. For additional information, please contact ELITechGroup. AquaPhluor® is a registered trademark of ELITechGroup.

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-5926-95 50 µmol 0.06 0.5 3.33 2 1.25 0.91 0.67 0.17
10-5926-90 100 µmol 0.13 1 20 12 7.5 5.45 4 1
10-5926-02 0.25 g 0.25 1.96 52 31.2 19.5 14.18 10.4 2.6

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-5926-95E 50 µmol 0.06 0.75 0.07 8.6 5.38 3.91 0.54
10-5926-90E 100 µmol 0.13 1.5 0.07 23.6 14.75 10.73 1.48
10-5926-02E 0.25 g 0.25 2.93 0.07 52.2 32.63 23.73 3.26