In oligonucleotide applications, there is often a need to introduce blocking groups on the 3’-end and sometimes at the 5’-end as well. These blocking groups can provide exonuclease protection or prevent polymerase extension. One such blocking group is an inverted dSpacer, which can be incorporated with dSpacer-5’-CE Phosphoramidite during standard 3’ to 5’ syntheses.
Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Freezer storage, -10 to -30°C, dry
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.