dT-5'-CE Phosphoramidite

3'-Dimethoxytrityl-2'-deoxyThymidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
main product photo

Product Specifications

Formula:
C40H49N4O8P
M.W.:
744.83
F.W.:
304.20
CAS Number:
134031-86-0
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet

Glen Report 6.14: 5' To 3' Synthesis

Glen Report 31.13: New Product - dmf-dG-5’-CPG

Description

Glen Research 5'-CE (ß-cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for structural studies.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability Similar to dA,C,G,T-CE Phosphoramidites

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-0301-02 0.25 g .25grams 3.36 98.67 59.2 37 26.91 19.73 4.93
10-0301-05 0.5 g .5grams 6.71 210.33 126.2 78.88 57.36 42.07 10.52
10-0301-10 1.0 g 1grams 13.43 434.33 260.6 162.88 118.45 86.87 21.72

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-0301-02 0.25 g .25grams 5.01 0.07 93.8 58.63 42.64 5.86
10-0301-05 0.5 g .5grams 10.02 0.07 194 121.25 88.18 12.13
10-0301-10 1.0 g 1grams 20.04 0.07 394.4 246.5 179.27 24.65

References

Synergistic effects on mutagenicity of tandem lesions containing 8-oxo-7,8-dihydro-2’-deoxyguanosine or Fapy dG flanked by a 3’- 5-formyl-2’-deoxyuridine in human cells Jan Henric T. Bacurio, Shijun Gao, Haozhe Yang, Ashis K. Basu, Marc M. Greenberg

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