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dSpacer CE Phosphoramidite

5'-O-Dimethoxytrityl-1',2'-Dideoxyribose-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

main product photo

Product Specifications

Formula:

C₃₅H₄₅N₂O₆P

M.W.:

620.73

F.W.:

180.10

CAS Number:

129821-76-7

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Technical Documents

Dilution/Coupling Data

Technical Documents

Safety Data Sheet (North America)

Safety Data Sheet (EU)

Safety Data Sheet (Other)

Glen Report 16.13: Minor Base and Related Novel Phosphoramidites

Glen Report 7.16: New Modification Reagents and Supports

Glen Report 35-13: New Product — dSpacer-5’-CE Phosphoramidite (Reverse dSpacer)

Glen Report 35-14: Technical Note — Purification of 5’-Labeled Oligonucleotides Using Glen-Pak DNA Purification Cartridges

Description

The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3'-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3'-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Storage	Freezer storage, -10 to -30°C, dry
Stability	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1914-02	0.25 g	.25grams	4.03	121	72.6	45.38	33	24.2	6.05
10-1914-90	100 µmol	.062grams	1	20	12	7.5	5.45	4	1

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1914-02	0.25 g	.25grams	6.01	0.07	113.8	71.13	51.73	7.11
10-1914-90	100 µmol	.062grams	1.5	0.07	23.6	14.75	10.73	1.48

References

Investigating HIV-1 Polypurine Tract Geometry via Targeted Insertion of Abasic Lesions in the(-)-DNA Template and (+)-RNA Primer Hye Young Yi-Brunozzi and Stuart F. J. Le Grice

Examining Ty3 Polypurine Tract Structure and Function by Nucleoside Analog Interference Chandravanu Dash, John P. Marino, and Stuart F. J. Le Grice Pedersen

Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs John P. McDonald, Ashley Hall, Didier Gasparutto, Jean Cadet, Jack Ballantyne, and Roger Woodgate

Redox signaling between DNA repair proteins for efficient lesion detection Amie K. Boal, Joseph C. Genereux, Pamela A. Sontz, Jeffrey A. Gralnick, Dianne K. Newman, and Jacqueline K. Barton

Incorporating 2-thiouracil into short dsRNA-binding PNAs for enhanced recognition of A-U pairs and for targeting a microRNA hairpin precursor Ong, A. A. L. et al.

Sequencing of Phosphoramidate Oligonucleotides by Acid Hydrolysis and Mass Spectrometry Pourshahian, S. & Gryaznov, S.

DNA Base Excision Repair Intermediates Influence Duplex-Quadruplex Equilibrium Mark L. Sowers, James W. Conrad, Bruce Chang-Gu, Ellie Cherryhomes, Linda C. Hackfeld, and Lawrence C. Sowers