Oligonucleotide Labeling

Glen Research labeling products are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by an Analytical Report and an HPLC trace, showing the results of our QC testing. Glen Research monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination.

Explore Label Phosphoramidites and Supports

Acridine phosphoramidite is designed to produce an oligonucleotide containing acridine at any position in the molecule. Acridine CPG is used to label the 3'-terminus. Acridine is an effective intercalating agent.

Our products for biotin labeling of synthetic oligonucletides are produced and packaged to ensure the highest performance on commercial synthesizers.

Synthetic oligonucleotides with covalently-attached CDPI3 have enhanced DNA affinity and have improved the hybridization properties of sequence-specific DNA probes.

We have designed our Cholesteryl products with triethyleneglycol (TEG) spacers for maximum solubility.

Cyclooctatetraene (COT) Serinol Phosphoramidite provides a means to improve the photostability of virtually any fluorophore in a modular fashion.

EDTA-C2-dT phosphoramidite contains the triethyl ester of EDTA which allows sequence-specific cleavage of single- and double-stranded DNA and RNA.

With an excellent stability profile, ferrocene has always attracted considerable interest for DNA labelling to generate probes for electrochemical detection.

Many different fluorophores can be readily attached to oligonucleotides to create fluorescent probes, which form the basis of the detection systems used in DNA sequencing, forensic science and genetic analysis.

Glen Research is delighted to introduce a GalNAc modification strategy using a monomeric GalNAc support and the equivalent GalNAc phosphoramidite.

Dimethylaminophenothiazin-5-ium chloride, Methylene Blue (MB), has many uses in chemistry and biology.

Psoralen C2 at the 5'-terminus of an oligonucleotide serves effectively as a cross-linking reagent in double-stranded oligonucleotides.

We now offer a simple C18 lipid as an economical and effective carrier molecule. We envisage that the 5'-stearyl group will become a favored lipophilic carrier for experimentation with synthetic oligonucleotides.

As an addition to our cholesteryl product line, we offer simple α-tocopheryl (vitamin E) labelling. Vitamin E is both lipophilic and non-toxic even at high doses so would be an excellent candidate as a lipophilic carrier for oligonucleotide

Glen Research offers a line of products based on the serinol backbone, which have been developed in close collaboration between Glen Research and Nelson Biotechnologies

Glen Research’s agreement with ELITechGroup, formerly Epoch Biosciences, allows us to offer several of their proprietary products designed for the synthesis of novel DNA probes.

Two cyanine derivatives, Cyanine 3 and Cyanine 5, which differ in structure simply by the number of carbons in the conjugated polyene linkage, are joined by the closely related analogues, Cyanine 3.5 and Cyanine 5.5, and are available as phosphoramidites.

The Dabcyl group has been found to be a universal quencher. 3’-Dabsyl CPG and 3’-Dabcyl CPG are used to prepare probes with the quencher blocking the 3’-terminus.

With the growing popularity of red and near-infrared dyes, we are offering the Black Hole QuencherTM dyes (BHQs).

We are happy to offer several products containing the BlackBerry® Quencher (BBQ-650®), which exhibits a broad absorption profile from 550nm to 750nm, centered at 650nm.

We offer 3’-TAMRA CPG for 3’ labeling and TAMRA-dT for labeling within the sequence. We also offer TAMRA NHS ester for labeling amino-modified oligonucleotides.

We have chosen the branched triethylene glycol (TEG) spacer in our version of DNP phosphoramidite since it can be added once or several times to the 3’ or 5’ terminus.

2,2’-Dipicolylamine Phosphoramidite has been discontinued This product was manufactured and developed by Syntrix Biosystems Inc.

Pyrene and perylene are fluorescent polycyclic aromatic hydrocarbons that have the ability to form ‘excited state dimers’ known as excimers.

Photocaged oligonucleotides allow for the spatial temporal control of biological processes.

Photo-control, the use of ultraviolet or visible light to control a reaction, has a number of advantages over other external stimuli:

When 3-cyanovinylcarbazole nucleoside (CNVK) is incorporated into an oligonucleotide, very rapid photo cross-linking to the complementary strand can be induced at one wavelength and rapid reversal of the cross-link is possible at a second wavelength.

QUAL probes consist of two oligonucleotides, the first containing a nucleophilic group at the 3’-terminus, while the second has an electrophilic group at the 5’-terminus.

Dichloro-diphenyl-fluorescein, SIMA (HEX) exhibits virtually identical absorbance and emission spectra to HEX.

Thiazole orange is an asymmetric cyanine dye whose fluorescence can be quite dependent on its local environment.

Puromycin binds in the ribosome’s A site, forms a peptide bond with the growing peptide chain, and blocks further peptide elongation. By linking puromycin to mRNA, a peptide-RNA fusion product results from the translation of the message linking the encoding mRNA with its peptide product.

Palmitate Phosphoramidite is a saturated fatty acid with a 16-carbon backbone. This 5’-modification is a lipophilic group. When incorporated into a synthetic oligonucleotide, Palmitate conjugates offer enhanced cellular uptake and delivery to extrahepatic tissues.