ELITechGroup Dyes and Quenchers

Glen Research’s agreement with ELITechGroup, formerly Epoch Biosciences, allows us to offer several of their proprietary products designed for the synthesis of novel DNA probes.  We are pleased to offer products based on ELITechGroup’s Redmond Red®, Yakima Yellow® and AquaPhluor® 593 fluorophores and Eclipse® non-fluorescent quencher.  Under our agreement we also supply PPG, a modified nucleoside, and 5’-Aldehyde-Modifier C2 Phosphoramidite.  The fluorescent dyes, Yakima Yellow, Redmond Red and AquaPhluor 593, are available as phosphoramidites and supports.  Yakima Yellow has an absorbance maximum at 530 nm and emission maximum at 549 nm, Redmond Red’s absorbance and emission maxima are at 579 nm and 595 nm, respectively, and AquaPhluor 593 has an absorbance maximum at 593 nm and emission maximum at 613 nm.   

The Eclipse quencher from ELITechGroup solves most of the problems inherent in the synthesis of molecular beacon and FRET probes.  The Eclipse molecule is highly stable and can be used safely in all common oligo deprotection schemes.  The absorbance maximum for Eclipse Quencher is at 522 nm, compared to 479 nm for dabcyl.  In addition, the structure of the Eclipse Quencher is substantially more electron deficient than that of dabcyl and this leads to better quenching over a wider range of dyes, especially those with emission maxima at longer wavelengths (red shifted) such as Redmond Red and Cyanine 5.  In addition, with an absorption range from 390 nm to 625 nm, the Eclipse Quencher is capable of effective performance in a wide range of colored FRET probes.

The spectral characteristics of these dyes are detailed here.

11 Product Results
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  • 5'-AquaPhluor® 593 CE Phosphoramidite
    5'-AquaPhluor® 593 CE Phosphoramidite (10-5923)
    Catalog #: 10-5923
    Formal Name:

    6-[2,3,6,7,12,13,16,17-Octohydro-1H,5H,11H,15H-Xantheno[2,3,4-ij:5,6,7-i’j’]diquinolizin-18-ium-9-yl-[(2-diethylaminocarbonyl)-phen-4-yl][trifluoroacetylaminobutoxy]phosphoryl][oxy]]-hexyl-1-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite,hexafluorophosphate salt

  • 5'-AquaPhluor® 639 CE Phosphoramidite
    5'-AquaPhluor® 639 CE Phosphoramidite (10-5926)
    Catalog #: 10-5926
    Formal Name: 6-[2,3,16,17-tetrahydro-5,5,7,11,13,13-hexamethyl-1H,5H,13H,15H- Xantheno[2,3,4-ij:5,6,7-i'j′]diquinolizin-18-ium-9-yl-[2-chloro-6- (N-hydroxysulfonylethyl-N’-methylaminocarbonyl)-phen-4-yl] [trifluoroacetylaminobutoxy]phosphoryl][oxy]-hexyl-1-O- (2-cyanoethyl)-(N,N-diisopropyl)-phosphoramidite
  • AquaPhluor® 593 CPG
    AquaPhluor® 593 CPG (20-5923)
    Catalog #: 20-5923
    Formal Name: 2,3,6,7,12,13,16,17-Octohydro-1H,5H,11H,15H-Xantheno[2,3,4-ij:5,6,7-i’j’]diquinolizin-18-ium-9-yl-[(2-diethylaminocarbonyl)-phen-4-yl]-phosphoryl-hexanoyl-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG
  • AquaPhluor® 639 CPG
    AquaPhluor® 639 CPG (20-5926)
    Catalog #: 20-5926
    Formal Name: 6-[2,3,16,17-tetrahydro-5,5,7,11,13,13-hexamethyl-1H,5H,13H,15H- Xantheno[2,3,4-ij:5,6,7-i'j′]diquinolizin-18-ium-9-yl-[2-chloro-6-(N- hydroxysulfonylethyl-N’-methylaminocarbonyl)-phen-4-yl] [trifluoroacetylaminobutoxy]phosphoryl][oxy]- hexanoyl-1-(2-O- dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG
  • Eclipse® Quencher CPG
    Eclipse® Quencher CPG (20-5925)
    Catalog #: 20-5925
    Formal Name: 4-N-methyl-N-(4'-nitro-2'-chloroazobenzen-4-yl)-aminobutanamido-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG
  • Eclipse® Quencher Phosphoramidite
    Eclipse® Quencher Phosphoramidite (10-5925)
    Catalog #: 10-5925  |  CAS #: 916753-64-5
    Formal Name: 4-N-methyl-N-(4'-nitro-2'-chloroazobenzen-4-yl)-aminobutanamido-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-O-(2-cyanoethyl)-(N,N-diisopropyl)-phosphoramdite
  • MGB Eclipse® CPG
    MGB Eclipse® CPG (20-5927)
    Catalog #: 20-5927
    Formal Name: 5-(6-(6-(6-(4-N-(3-Dimethoxytrityloxypropyl)-N-(4'-nitro-2'-chloroazobenzen-4-yl)-aminobutanoyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carbonyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carbonyl)-3,6,7,8-tetrahydropyrrolo[3,2-e]indole-2-carboxamido)pentyl-1-O-diglycoloyl long chain alkylamino CPG
  • Redmond Red® CPG
    Redmond Red® CPG (20-5920)
    Catalog #: 20-5920
    Formal Name:

    3-(3-oxo-7-pivaloylphenoxazin-2-yl)-propanamido-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG

  • Redmond Red® Phosphoramidite
    Redmond Red® Phosphoramidite (10-5920)
    Catalog #: 10-5920
    Formal Name:


  • Yakima Yellow® CPG
    Yakima Yellow® CPG (20-5921)
    Catalog #: 20-5921
    Formal Name:

    3-(5,6,4',7'-tetrachloro-5'-methyl-3',6'-dipivaloylfluorescein-2-yl)-propanamido-1-(2-O-dimethoxytrityloxymethyl)-pyrrolidin-4-yl-succinoyl long chain alkylamino-CPG

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