Rhodamine (TAMRA) Labeling of Oligonucleotides

Tetramethylrhodamine (TAMRA) is a fluorescent dye that has been popularly used for labeling oligonucleotides. Because of its instability to ammonium hydroxide, TAMRA was first used as TAMRA NHS Ester to label amino-modified oligonucleotides. However, later work showed that TAMRA is stable to UltraMild deprotection and also deprotection with hindered amines, like t-buylamine, so supports and phosphoramidites containing TAMRA were quickly introduced.  TAMRA-modified oligonucleotides have established an important role in both Förster resonance energy transfer (FRET) and real-time PCR applications.

The spectral characteristics of these dyes are detailed here.

5 Product Results
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  • ROX NHS Ester
    ROX NHS Ester (50-5911)
    Catalog #: 50-5911  |  CAS #: 1890922-83-4
    Formal Name: 6-Carboxy-X-rhodamine, N-hydroxysuccinimide ester
  • TAMRA-dT
    TAMRA-dT (10-1057)
    Catalog #: 10-1057  |  CAS #: 1266091-48-8
    Formal Name:


  • TAMRA NHS Ester
    TAMRA NHS Ester (50-5910)
    Catalog #: 50-5910  |  CAS #: 150810-69-8
    Formal Name:

    Carboxytetramethylrhodamine, N-hydroxysuccinimide ester in dimethyl sulfoxide (DMSO)

  • 3'-TAMRA CPG
    3'-TAMRA CPG (20-5910)
    Catalog #: 20-5910
    Formal Name:

    1-Dimethoxytrityloxy-3-[O-(N-carboxy-(Tetramethyl-rhodamine)-3-aminopropyl)]-propyl-2-O-succinoyl-long chain alkylamino-CPG

  • 3'-TAMRA PS
    3'-TAMRA PS (26-5910)
    Catalog #: 26-5910
    Formal Name:


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