EDTA Labeling of Oligonucleotides

EDTA-C2-dT phosphoramidite contains the triethyl ester of EDTA which allows sequence-specific cleavage of single- and double-stranded DNA and RNA.  The cleavage reaction is only initiated once Fe(II) and dithiothreitol are added and so is readily controlled.   Coupling of EDTA-dT is normal but cleavage and deprotection should be carried out with sodium hydroxide in aqueous methanol (0.4M NaOH in methanol/water 4:1) overnight at room temperature.

1 Product Results
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  • EDTA-C2-dT-CE Phosphoramidite
    EDTA-C2-dT-CE Phosphoramidite (10-1059)
    Catalog #: 10-1059
    Formal Name: 5'-Dimethoxytrityl-5-[N-ethylenediaminetriethylacetate,monoacetylaminoethyl-3-acrylimido]-2'-deoxyUridine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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