Glen Research phosphoramidites and supports for DNA modification are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by an Analytical Report and an HPLC trace, showing the results of our QC testing. Glen Research monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination.
Aldehyde modifiers would be attractive electrophilic substitutions in oligonucleotides since they are able to react with amino groups to form a Schiff's base, with hydrazino groups to form hydrazones, and with semicarbazides to form semi-carbazones.
Glen Research 5'-Modifiers are designed for use in DNA synthesizers to functionalize the 5'-terminus of the target oligonucleotide. 3'-Amino-Modifier CPGs, containing amino groups protected with the base-labile Fmoc group, are designed to functionalize the 3'-terminus of the target oligonucleotide by the introduction of a primary amine.
The simplest approach to carboxy modification of oligos is to include the carboxylate NHS ester in the phosphoramidite to form the protected and activated carboxylate in situ. Both of our NHS ester carboxy-modifiers (5'-Carboxy-Modifier C10 and NHS-Carboxy-dT) use this approach.
We offer a variety of products to phosphorylate the 3' or the 5'-terminus of an oligonucleotide during synthesis.
With Glyceryl CPG the 3'-terminus of an oligonucleotide is readily oxidized by sodium periodate to form a 3'-phosphoglycaldehyde.
Maleimide Modifier Phosphoramidite can be incorporated into DNA and RNA with both phosphate and phosphorothioate linkages.
Our photocleavable (PC) phosphoramidites are produced and packaged to ensure the highest performance on commercial oligonucleotide synthesizers.
Our various spacer modifier phosphoramidites and supports are produced and packaged to ensure the highest performance on commercial oligonucleotide synthesizers.
Thiol-modification of oligonucleotides is important for: 1) Labeling with thiol-specific tags like iodoacetamides and maleimides, 2) Conjugation of enzymes, especially horseradish peroxidase, and 3) Attachment of oligonucleotides to gold surfaces.
Glen Research offers a variety of supports for the 3'-modification of oligonucleotides.
Glen Research offers a variety of phosphoramidites for the 5'-modification of oligonucleotides.