Glen Research CE (β-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing.Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination, and each vial type is thoroughly tested or inspected to ensure a tight fit on synthesizers.
All Glen Research CPG supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.
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Oligonucleotide synthesis is routinely carried out from the 3' to the 5' terminus for no other reason than the ease of synthesis of the monomer units. Glen Research offers phosphoramidites and supports for backwards oligonucleotide synthesis.
Depurination is defined as the cleavage of the glycosidic bond attaching a purine base to the sugar moiety.Depurination resistant monomers are available.
Our standard base protection phosphoramidites and support are produced and packaged to ensure the highest performance on commercial oligonucleotide synthesizers.
All of the trimers are available individually so the researchers can prepare custom trimer mixes. Two pre-made catalog trimer mixes are also available
UltraMild DNA synthesis reagents are available for use when DNA bases and labels are not compatible with ammonium hydroxide deprotection.
Glen Research offers DNA Phosphoramidite Mixes for the synthesis of degenerate oligonucleotides.