2’-OMe-N6-Me-A, commonly referred to as m6Am, is a crucial component of the 5’-cap of mRNA. 2’-OMe-A is often the first transcribed nucleotide, and this modification is a result of methylation at the N6-position of the nucleobase. The 5’-cap structures initiate translation and protect RNA from degradation. Internal m6Am, have also been implicated in regulating gene expression, RNA splicing, and cancer proliferation. We offer the 2’-OMe-N6-Me-A RNA monomer with a phenoxyacetyl protecting group to minimize potential branching. 2’-OMe-N6-Me-A-CE Phosphoramidite is compatible with popular synthesis and deprotection methods, from UltraMild to AMA.
Details
Usage
Coupling: 6 minute coupling time. To avoid any exchange of the iPr-Pac group on the G with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
Deprotection: UltraMild deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium Hydroxide.
Specifications
Diluent
Anhydrous Acetonitrile
Storage
Freezer storage, -10 to -30°C, dry
Stability
1-2 Days
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
