TOM-Protected Phosphoramidites for RNA synthesis

RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection.  High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers.  Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature.  A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration.  This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group.  These features allow the TOM-Protected monomers to produce longer oligonucleotides.  TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.

INTELLECTUAL PROPERTY

TOM-Protecting-Group is a trademark of QIAGEN.