TOM-Protected RNA Phosphoramidites

RNA synthesis using monomers containing the 2’-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection.  High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2’-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers.  Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature.  A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2’ to 3’ migration.  This migration under basic conditions leads to non-biologically active 2’-5’ linkages when using the TBDMS group.  These features allow the TOM-Protected monomers to produce longer oligonucleotides.  TOM-Protected RNA monomers are also fully compatible with minor bases with 2’-O-TBDMS protection.


TOM-Protecting-Group is a trademark of QIAGEN.  

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  • A-TOM-CE Phosphoramidite
    A-TOM-CE Phosphoramidite (10-3004)
    Catalog #: 10-3004
    Formal Name: 5'-Dimethoxytrityl-N-acetyl-Adenosine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • C-TOM-CE Phosphoramidite
    C-TOM-CE Phosphoramidite (10-3014)
    Catalog #: 10-3014
    Formal Name: 5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • G-TOM-CE Phosphoramidite
    G-TOM-CE Phosphoramidite (10-3024)
    Catalog #: 10-3024
    Formal Name: 5'-Dimethoxytrityl-N-acetyl-Guanosine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
  • U-TOM-CE Phosphoramidite
    U-TOM-CE Phosphoramidite (10-3034)
    Catalog #: 10-3034
    Formal Name:


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