5'-Dimethoxytrityl-Uridine,2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
RNA synthesis using monomers containing the 2'-O-TriisopropylsilylOxyMethyl (TOM) group (TOM-Protecting-Group™) is characterized by very high coupling efficiency along with fast, simple deprotection. High coupling efficiency is achieved because the TOM-Protecting-Group exhibits lower steric hindrance than the 2'-O-t-butyldimethylsilyl (TBDMS) group used in our alternative RNA monomers. Fast and reliable deprotection is achieved using methylamine in ethanol/water at room temperature. A further feature of the TOM-Protecting-Group is that during basic steps it can not undergo 2' to 3' migration. This migration under basic conditions leads to non-biologically active 2'-5' linkages when using the TBDMS group. These features allow the TOM-Protected monomers to produce longer oligonucleotides. TOM-Protected RNA monomers are also fully compatible with minor bases with 2'-O-TBDMS protection.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Freezer storage, -10 to -30°C, dry |
| Stability | 24 hours |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-3034-02 | 0.25 g | 0.25grams | 2.68 | 76 | 45.6 | 28.5 | 20.73 | 15.2 | 3.8 |
| 10-3034-05 | 0.5 g | 0.5 grams | 5.36 | 165.33 | 99.2 | 62 | 45.09 | 33.07 | 8.27 |
| 10-3034-10 | 1.0 g | 1grams | 10.72 | 344 | 206.4 | 129 | 93.82 | 68.8 | 17.2 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-3034-02 | 0.25 g | 0.25grams | 4 | 0.07 | 73.6 | 46 | 15.33 | 4.6 |
| 10-3034-05 | 0.5 g | 0.5 grams | 8 | 0.07 | 153.6 | 96 | 32 | 9.6 |
| 10-3034-10 | 1.0 g | 1grams | 15.99 | 0.07 | 313.4 | 195.8 | 65.29 | 19.5 |
