5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-uridine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability | 1-2 Days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-3231-05 | 0.5 g | .5grams | 6.11 | 190.33 | 114.2 | 71.38 | 51.91 | 38.07 | 9.52 |
| 10-3231-10 | 1.0 g | 1grams | 12.21 | 393.67 | 236.2 | 147.63 | 107.36 | 78.73 | 19.68 |
| 10-3231-20 | 2.0 g | 2grams | 24.42 | 800.67 | 480.4 | 300.25 | 218.36 | 160.13 | 40.03 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-3231-05E | 0.5 g | .5grams | 9.11 | 0.07 | 175.8 | 109.88 | 79.91 | 10.99 |
| 10-3231-10E | 1.0 g | 1grams | 0 | 0.07 | 358.2 | 223.88 | 162.82 | 22.39 |
| 10-3131-20E | 2.0 g | 2grams | 0 | 0.07 | 722.6 | 451.63 | 328.45 | 45.16 |
