5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-N6-benzoyl-adenosine -3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability | 1-2 Days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-3200-05 | 0.5 g | 0.5grams | 5.3 | 165.33 | 99.2 | 62 | 45.09 | 33.07 | 8.27 |
| 10-3200-10 | 1.0 g | 1grams | 10.73 | 344.33 | 206.6 | 129.13 | 93.91 | 68.87 | 17.22 |
| 10-3200-20 | 2.0 g | 2grams | 21.46 | 702 | 421.2 | 263.25 | 263.25 | 140.4 | 35.1 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-3200-05E | 0.5 g | 0.5grams | 8.01 | 0.07 | 153.8 | 96.13 | 69.91 | 9.61 |
| 10-3200-10E | 1.0 g | 1grams | 16.01 | 0.07 | 313.8 | 196.13 | 142.64 | 19.61 |
| 10-3200-20E | 2.0 g | 2grams | 32.03 | 0.07 | 634.2 | 396.38 | 288.27 | 39.64 |
