5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-5-methyl-N4-benzoyl- cytidine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability | 1-2 Days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-3211-05 | 0.5 g | 0.5grams | 5.42 | 167.33 | 100.4 | 62.75 | 45.64 | 33.47 | 8.37 |
| 10-3211-10 | 1.0 g | 1grams | 10.85 | 348.33 | 209 | 130.63 | 95 | 69.67 | 17.42 |
| 10-3211-20 | 2.0 g | 2grams | 21.69 | 709.67 | 425.8 | 266.13 | 193.55 | 141.93 | 35.48 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-3211-05E | 0.5 g | 0.5grams | 8.09 | 0.07 | 155.4 | 97.13 | 70.64 | 9.71 |
| 10-3211-10E | 1.0 g | 1grams | 16.19 | 0.07 | 317.4 | 198.38 | 144.27 | 19.84 |
| 10-3211-20E | 2.0 g | 2grams | 32.38 | 0.07 | 641.2 | 400.75 | 291.45 | 40.08 |
