5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-N2-isobutyryl- guanosine-3'-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Like the very similar 2’-OMe backbone, the 2’-O-methoxyethyl-RNA (2’-MOE) backbone provides enhanced duplex stability, significant nuclease resistance and relatively low toxicity. As a result, 2’-MOE has been an attractive backbone for many therapeutic candidates, several of which have been approved by the FDA. These drugs have included 1) 2’-MOE/DNA chimeras to facilitate RNase H cleavage of target RNA sequences as well as 2) steric blocking oligonucleotides to alter the splicing of mRNA. The standard 2’-MOE nucleotides are A, 5-Me-C, G and 5-Me-U.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability | 1-2 Days |
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-3220-05 | 0.5 g | .5grams | 5.47 | 169 | 1.4 | 63.38 | 46.09 | 33.8 | 8.45 |
| 10-3220-10 | 1.0 g | 1grams | 10.94 | 351.33 | 210.8 | 131.75 | 95.82 | 70.27 | 17.57 |
| 10-3220-20 | 2.0 g | 2grams | 21.88 | 716 | 429.6 | 268.5 | 195.27 | 143.2 | 35.8 |
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-3220-05E | 0.5 g | .5grams | 8.16 | 0.07 | 156.8 | 98 | 71.27 | 9.8 |
| 10-3220-10E | 1.0 g | 1grams | 16.33 | 0.07 | 320.2 | 200.13 | 145.55 | 20.01 |
| 10-3220-20E | 1 g | 2grams | 32.66 | 0.07 | 646.8 | 404.25 | 294 | 40.43 |
