2'-OMe-G-CE Phosphoramidite

5'-Dimethoxytrityl-N-dimethylaminomethylidene-Guanosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
main product photo

Product Specifications

Formula:
C44H55N8O8P
M.W.:
854.93
F.W.:
359.24
CAS Number:
128219-77-2
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Aptamers as Drugs

Safety Data Sheet

Glen Report 6.12: 2'-OMe-RNA Update

Glen Report 6.23: Research Review - Antisense RNA

Glen Report 5-11: 2'-0Me-RNA -The Logical RNA Alternative?

Glen Report 4-13: RNA Synthesis - 2'-O-Methyl Analogues

Glen Report 4-14: Literature Review

Glen Report 35-16: Technical Note — Sugar Conformations and Modifications

Description

Glen Research 2'-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Details

Usage

  • Coupling: 6 minute coupling time recommended
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 3-5 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3121-02 0.25 g .25grams 2.92 84 50.4 31.5 22.91 16.8 4.2
10-3121-05 0.5 g .5grams 5.85 181.67 109 68.13 49.55 36.33 9.08
10-3121-10 1.0 g 1grams 11.7 376.67 226 141.25 102.73 75.33 18.83
10-3121-90 100 µmol .085grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3121-02 0.25 g .25grams 4.36 0.07 80.8 50.5 36.73 5.05
10-3121-05 0.5 g .5grams 8.73 0.07 168.2 105.13 76.45 10.51
10-3121-10 1.0 g 1grams 17.46 0.07 342.8 214.25 155.82 21.43
10-3121-90 100 µmol .085grams 1.5 0.07 23.6 14.75 10.73 1.48

References

Three essential and conserved regions of the group II intron are proximal to the 5'-splice site Alexandre de Lencastre and Anna Marie Pyle

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