6-Thioguanosine (6-thio-G) has applications in ribozyme and siRNA research, as well as in RNA-protein interactions. The removal of the silyl protecting group without interfering with the sulfur is critical. This is removed1 cleanly by triethylamine trihydrofluoride in DMSO but t-butylammonium fluoride (TBAF) leads to degradation of the thio-nucleotide analogue and should not be used.
Details
Usage
Coupling: 12 minute coupling time recommended. Use 0.02M Iodine to avoid degradation and Ac-C (10-3015).
Deprotection: Treat column with 1M DBU in ACN for 4 hrs at RT, rinse and dry support. Then deprotect with 50mM NaSH in AMA at 65°C for 10 minutes. Complete using RNA deprotection. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
Specifications
Diluent
Anhydrous Acetonitrile
Storage
Refrigerated storage, maximum of 2-8°C, dry
Stability
2-3 days
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
Catalog #
Pack Size
Grams/Pack
0.1M Dil. (mL)
Approximate Number of Additions
LV40
LV200
40nm
0.2μm
1μm
10μm
10-3072-02
0.25 g
.25grams
2.41
67
40.2
25.13
18.27
13.4
3.35
10-3072-90
100 µmol
.104grams
1
20
12
7.5
5.45
4
1
10-3072-95
50 µmol
.052grams
0.5
3.33
2
1.25
0.91
0.67
0.17
Expedite
Catalog #
Pack Size
Grams/Pack
Dilution (mL)
Approximate Number of Additions
Molarity
50nm
0.2μm
1μm
15μm
10-3072-02
0.25 g
.25grams
3.59
0.07
65.4
40.8
13.63
4
10-3072-90
100 µmol
.104grams
1.5
0.07
23.6
14.7
4.92
1.4
10-3072-95
50 µmol
.052grams
0.75
0.07
8.6
5.3
1.79
0.5
References
REFERENCE(S)| 1 C.J. Adams, J.B. Murray, M.A. Farrow, J.R.P. Arnold, and P.G. Stockley, Tetrahedron Lett., 1995, 36, 5421-5424.| 2 D.A. Berry, et al., Tetrahedron Lett, 2004, 45, 2457-2461.
