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5-Me-U-CE Phosphoramidite
5'-Dimethoxytrityl-5-Methyl-Uridine, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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Description
Inosine and 5-Methyl-Uridine are useful for analyzing RNA structure and activity relationships.
Details
Usage
- Coupling: 12 minute coupling time recommended
- Deprotection: RNA deprotection. See Technical Bulletin for details (http://www.glenresearch.com/Technical/TB_RNA_TBDMS_Deprotection.pdf).
| Specifications |
| Diluent |
Anhydrous Acetonitrile |
| Storage |
Freezer storage, -10 to -30°C, dry |
| Stability |
2-3 days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # |
Pack Size |
Grams/Pack |
0.1M Dil. (mL) |
Approximate Number of Additions |
|
LV40 |
LV200 |
40nm |
0.2μm |
1μm |
10μm |
| 10-3050-02 |
0.25 g |
.25grams |
2.86 |
82 |
49.2 |
30.75 |
22.36 |
16.4 |
4.1 |
| 10-3050-90 |
100 µmol |
.088grams |
1 |
20 |
12 |
7.5 |
5.45 |
4 |
1 |
| 10-3050-95 |
50 µmol |
.044grams |
0.5 |
3.33 |
2 |
1.25 |
0.91 |
0.67 |
0.17 |
Expedite
| Catalog # |
Pack Size |
Grams/Pack |
Dilution (mL) |
Approximate Number of Additions |
|
Molarity |
50nm |
0.2μm |
1μm |
15μm |
| 10-3050-02 |
0.25 g |
.25grams |
4.26 |
0.07 |
78.8 |
49.2 |
16.42 |
4.9 |
| 10-3050-90 |
100 µmol |
.088grams |
1.5 |
0.07 |
23.6 |
14.7 |
4.92 |
1.4 |
| 10-3050-95 |
50 µmol |
.044grams |
0.75 |
0.07 |
8.6 |
5.3 |
1.79 |
0.5 |
