Ac-dC-CE Phosphoramidite

5'-Dimethoxytrityl-N-acetyl-2'-deoxyCytidine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
main product photo

Product Specifications

Formula:
C41H50N5O8P
M.W.:
771.85
F.W.:
289.18
CAS Number:
154110-40-4
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet (North America)

Safety Data Sheet (EU)

Safety Data Sheet (Other)

Glen Report 11.25: Alternatives to Expedite Monomers

Glen Report 19.24: New Lines of Monomers - HT for High Throughput and LC for Low Cost RNA

Glen Report 26.14: Technical Brief - AMA – Ubiquitous Reagent for Oligo Deprotection

Glen Report 27.13: Technical Brief - APA: An Alternative to AMA Deprotection

Glen Report 6.22: UltraFast DNA Synthesis - 10 Minute Deprotection

Glen Report 7.12: UltraFast Chemistry

Description

Glen Research CE (B-cyanoethyl) Phosphoramidites are produced and packaged to ensure the highest performance on DNA synthesizers. Every Glen Research product is accompanied by a Certificate of Analysis and HPLC trace, showing the results of our QC testing. Every Glen Research monomer vial is specially cleaned to eliminate particulate contamination, and each vial type is thoroughly tested or inspected to ensure a tight fit on synthesizers.

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Specifications
Diluent Anhydrous Acetonitrile
Storage Controlled room temperature or lower, dry
Stability Similar to dA,C,G,T-CE Phosphoramidites

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1015-02 0.25 g .25grams 3.24 94.67 56.8 35.5 25.82 18.93 4.73
10-1015-05 0.5 g .5grams 6.48 202.67 121.6 76 55.27 40.53 10.13
10-1015-10 1.0 g 1grams 12.96 418.67 251.2 157 114.18 83.73 20.93
10-1015-20 2.0 g 2grams 25.91 850.33 510.2 318.88 231.91 170.07 42.52
10-1015-40 4.0 g 4grams 51.82 1714 1028.4 642.75 467.45 342.8 85.7

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1015-C2 0.25 g .25grams 4.83 0.07 93.4 58.38 42.45 5.84
10-1015-C5 0.5 g .5grams 9.67 0.07 190.2 118.88 86.45 11.89
10-1015-1C 1.0 g 1grams 19.34 0.07 383.6 239.75 174.36 23.98
10-1015-2C 2.0 g 2grams 38.67 0.07 770.2 481.38 350.09 48.14

References

Chemical synthesis of oligodeoxyribonucleotides containing the Dewar valence isomer of the (6-4) photoproduct and their use in (6-4) photolyase studies Junpei Yamamoto, Kenichi Hitomi, Takeshi Todo,and Shigenori Iwai

New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Förster resonance energy transfer probes in 5'-nuclease assays. Igor V. Kutyavin

Solid-Phase Synthesis of Oligodeoxyribonucleotides without Base Protection Utilizing O-Selective Reaction of Oxazaphospholidine Derivatives N. Oka, Y. Maizuru, M. Shimizu, and T. Wada

Thrombin binding aptamer G-quadruplex stabilized by pyrene-modified nucleotides Matic Kovačič, Peter Podbevšek, Hisae Tateishi-Karimata, Shuntaro Takahashi, Naoki Sugimoto, Janez Plavec,

Development of decoy oligonucleotide-warheaded chimeric molecules targeting STAT3 Po-Chang Shih, Miyako Naganuma, Genichiro Tsuji, Yosuke Demizu, and Mikihiko Naito

A unified Watson-Crick geometry drives transcription of six-letter expanded DNA alphabets by E.coli RNA polymerase Juntaek Oh, Zelin Shan, Shuichi Hoshika, Jun Xu, Jenny Chong, Steven A. Benner, Dmitry Lyumkis, and Dong Wang

Oligodeoxynucleotide Synthesis Under Non-Nucleophilic Deprotection Conditions Shiyue Fang, Komal Chillar, Yipeng Yin, Alexander Apostle, Dhananjani N. A. M. Eriyagama, Shahien Shahsavari, Bhaskar Halami, and Yinan Yuan

A multiplexed and quantitative assay for small-molecule detection via photo-crosslinking of structure switching aptamers Sharon S. Newman, Brandon Wilson, Liwei Zheng, Michael Eisenstein, Tom Soh

Selection of DNA aptamers that prevent the fibrillization of a-synuclein protein in cellular and mouse models Erin M. McConnell, Dennis Chan, Katelyn Ventura, Joshua P. Callahan, Kathryn Harris, Vernon H. Hunt, Spencer Boisjoli, Daniel Knight, Evan T. Monk, Matthew R. Holahan, and Maria C. DeRose.

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Product FAQs

  • How does this work? What would happen if I used the AMA reagent with Bz protected dC?
  • Can the AMA reagent be made up and stored? for how long? in what kind of container?