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Catalog Number: 10-1514-xx
Description: 5-Formyl-dC-CE Phosphoramidite
| 5'-Dimethoxytrityl-N-acetyl-5-(1,2-diacetyloxy-ethyl)-2'-deoxyCytidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C47H58N5O12P | M.W.: 915.96 | F.W.: 317.19 (formyl) FW: 349.23 (diol) |
Diluent: Anhydrous Acetonitrile |
| Coupling: 3 minute coupling time recommended |
| Deprotection: Deprotect with ammonium hydroxide for 17 hours at 55°C, AMA (40% aqueous methylamine/28% ammonium hydroxide (1:1)) for 10 minutes at 65°C, or 0.4 M NaOH in MeOH/water 4:1 (v/v) for 17 hours at room temperature. Oxidize with 50mM sodium periodate for 30 minutes at 4°C and desalt. See Technical Bulletin for details (Technical Bulletin).Note: NaOH is not compatible with dmf protecting groups. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 1-2 days |
| Catalog Information | Material Safety Data Sheet | Technical Notes |
Literature Highlights
| Glen Report 23.1: DNA Methylation Revisited |
| Glen Report 23.2: Glen Research Epigenetic Bases Report |
| Glen Report 24.2: Technical Brief - Improved Conditions for Deprotecting 5-Formyl-dC |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | 0.1M Dil. (mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1514-02 | 0.25grams | .25grams | 2.73 | 77.67 | 46.6 | 29.13 | 21.18 | 15.53 | 3.88 |
| 10-1514-90 | 100µmoles | .092grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1514-95 | 50µmoles | .046grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1514-02 | 0.25grams | .25grams | 4.07 | .07 | 75 | 46.88 | 34.09 | 4.69 | |
| 10-1514-90 | 100µmoles | .092grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1514-95 | 50µmoles | .046grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | Pack Size | Grams/ Pack | Dilution (mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1514-02 | 0.25grams | .25grams | 4.07 | .07 | 76.6 | 47.88 | 34.82 | ||
| 10-1514-90 | 100µmoles | .092grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1514-95 | 50µmoles | .046grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
01/15/2013 | http://www.glenres.com/ProductFiles/10-1514.html