Pyridin-2-one-CE Phosphoramidite

5'-Dimethoxytrityl-pyridin-2-one-riboside, 2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

This product has been discontinued.

We have discontinued this product permanently. Please find recommended products below.
main product photo

Product Specifications

Formula:
C46H62N3O8PSi
M.W.:
844.06
F.W.:
289.18
CAS Number:
179915-57-2
To Retrieve a Catalog Number, Select a Pack Size and Format:

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Technical Documents

Safety Data Sheet

TBDMS-Protected RNA Guide

Glen Report 19.11: Modified RNA Phosphoramidites Useful in siRNA Research and Biologically Significant 1-Methyl-adenosine

Description

Ribozyme activity is substantially affected by the substitution of modified pyrimidine bases. Zebularine (pyrimidin-2-one ribonucleoside) may be regarded as a Cytidine derivative lacking the exocyclic amino group. Zebularine and Pyridin-2-one Ribonucleoside, the 3-deaza analogue of Zebularine, are prime candidates for use in evaluating ribozyme activity and function. It should be noted that Zebularine is mildly fluorescent, absorbing at 298nm and emitting at 367nm.

Details

Usage

  • Coupling: 12 minute coupling time recommended.
  • Deprotection: RNA Deprotection-see bulletin
Specifications
Diluent Anhydrous Acetonitrile
Storage Freezer storage, -10 to -30°C, dry
Stability 2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-3012-02 0.25 g .25grams 2.96 85.33 51.2 32 23.27 17.07 4.27
10-3012-90 100 µmol .084grams 1 20 12 7.5 5.45 4 1
10-3012-95 50 µmol .042grams 0.5 3.33 2 1.25 0.91 0.67 0.17

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-3012-02 0.25 g .25grams 4.42 0.07 82 51.2 17.08 5.1
10-3012-90 100 µmol .084grams 1.5 0.07 23.6 14.7 4.92 1.4
10-3012-95 50 µmol .042grams 0.75 0.07 8.6 5.3 1.79 0.5