Etheno-dA-CE Phosphoramidite

5'-Dimethoxytrityl-etheno-deoxyAdenosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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Product Specifications

Formula:
C42H48N7O6P
M.W.:
777.86
F.W.:
337.23
CAS Number:
160702-08-9
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet

Extinction Table: Extinction Coefficient and Fluorescence Data

Glen Report 6.16: Etheno-dA and Etheno-A - Fluorescent Monomers

Glen Report 33-15: Technical Note - DNA and RNA Nucleoside Numbering System

Description

Etheno-dA is a fluorescent nucleoside which is especially useful in observing the transition between DNA structural types. It is quite base labile and should be deprotected with ammonium hydroxide at room temperature for 24 hours. Alternatively, UltraMild chemistry can be used. 2-Aminopurine and AP-dC (G-Clamp) are also useful fluorescent

Details

Usage

  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer. The use of UltraMILD monomers are preferred. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx). To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx).
  • Deprotection: If UltraMILD reagents were used, deprotect in 0.05M Potassium Carbonate in Methanol for 4 hours at Room Temperature OR for 2 hours at hours in 30% Ammonium Hydroxide. If standard bases were used, deprotection in Ammonium Hydroxide at Room Temperature for 24-36 hours will give acceptable yields.
Specifications
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry
Stability 1-2days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1006-02 0.25 g .25grams 3.21 93.67 56.2 35.13 25.55 18.73 4.68
10-1006-90 100 µmol .078grams 1 20 12 7.5 5.45 4 1

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1006-02 0.25 g .25grams 4.8 0.07 89.6 56 40.73 5.6
10-1006-90 100 µmol .078grams 1.5 0.07 23.6 14.75 10.73 1.48

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