DEACM Caged-dG-CE Phosphoramidite

5'-Dimethoxytrityl-N2-(4-isopropylphenoxyacetyl)-O6-[[7-(diethylamino)coumarin-4-yl]-methyl]-2'-deoxyGuanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
main product photo

Product Specifications

Formula:
C65H75N8O11P
M.W.:
1175.31
F.W.:
558.49 (DEACM-dG), 329.21 (dG)
CAS Number:
1325717-65-4
To Retrieve a Catalog Number, Select a Pack Size and Format:
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Technical Documents

Safety Data Sheet

Glen Report 34-11: Beyond the UV Region — DEACM-dG as a Versatile Tool for Light-Activatable (“Caged”) Oligonucleotides

Glen Report 34-12: New Product — DEACM Caged-dG

Description

Photocaged oligonucleotides allow for the spatial temporal control of biological processes. In their caged form, the oligonucleotides are unable to participate in base pairing. It is only after exposure to non-invasive light that the native oligonucleotide is released. DEACM-dG is a convenient way to introduce a photocage at specific positions, and the DEACM is an effective photocleavable group.

Details

Usage

  • Coupling: 3 minute coupling time recommended.
  • Deprotection: If UltraMILD reagents are used, deprotect in 0.05M Potassium Carbonate in Methanol overnight at Room Temperature. If standard bases are used, deprotect in Ammonium Hydroxide at Room Temperature overnight (dmf-dG required) or 30% ammonia/40% methylamine 1:1 (AMA) at Room Temperature for 2 hours (Ac-dC required). Heat should be avoided, both during deprotection and processing.
Specifications
Diluent Anhydrous Acetonitrile
Storage Storage: Freezer storage, -10 to -30°C, dry
Stability 1-2 Days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1533-95 50 µmol 0.06 0.5 3.33 2 1.25 0.91 0.67 0.17
10-1533-90 100 µmol 0.12 1 20 12 7.5 5.45 4 1
10-1533-02 0.25 g 0.25 2.13 57.67 34.6 21.63 15.73 11.53 2.88

Expedite

Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1533-95E 50 µmol 0.06 0.75 0.07 8.6 5.38 3.91 0.54
10-1533-90E 100 µmol 0.12 1.5 0.07 23.6 14.75 10.73 1.48
10-1533-02E 0.25 g 0.25 3.17 0.07 57 35.63 25.91 3.56

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