NPOM Caged-dT-CE Phosphoramidite

5'-Dimethoxytrityl-N3-[[1-(6-nitro-1,3-benzodioxol-5-yl)ethoxy]methyl]-2'-deoxyThymidine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Product Specifications

527.38 (N-POM-dT) FW: 304.20 (dT)
CAS Number:
To Retrieve a Catalog Number, Select a Pack Size and Format:


Photocaged oligonucleotides allow for the spatial temporal control of biological processes. In their caged form, the oligonucleotides are unable to participate in base pairing. It is only after exposure to non-invasive light that the native oligonucleotide is released. 

Glen Research’s interest lies in the preparation of caged oligonucleotides whose function is restored after uncaging by UV light at a wavelength that causes no DNA damage. The Deiters group at North Carolina State University has described NPOM-Caged-dT, where the nucleobase is caged with the photolabile group, 6-nitropiperonyloxymethyl (NPOM), which can be removed using UV light at 365 nm. Oligonucleotides containing NPOM-Caged-dT every five or six bases do not hybridize to their complementary strand. Photo-uncaging of the caged oligonucleotide is then easily carried out with UV light at 365 nm for seconds to minutes to restore the activity of the oligonucleotide



  • Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
  • Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
Diluent Anhydrous Acetonitrile
Storage Refrigerated storage, maximum of 2-8°C, dry. Light sensitive material.
Stability 1-2 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog # Pack Size Grams/Pack 0.1M Dil. (mL) Approximate Number of Additions
LV40 LV200 40nm 0.2μm 1μm 10μm
10-1534-02 0.25 g .25grams 2.58 72.67 43.6 27.25 19.82 14.53 3.63
10-1534-90 100 µmol .097grams 1 20 12 7.5 5.45 4 1
10-1534-95 50 µmol .048grams 0.5 3.33 2 1.25 0.91 0.67 0.17


Catalog # Pack Size Grams/Pack Dilution (mL) Approximate Number of Additions
Molarity 50nm 0.2μm 1μm 15μm
10-1534-02 0.25 g .25grams 3.85 0.07 70.6 44.13 32.09 4.41
10-1534-90 100 µmol .097grams 1.5 0.07 23.6 14.75 10.73 1.48
10-1534-95 50 µmol .048grams 0.75 0.07 8.6 5.38 3.91 0.54