Coumarin Azide


Product Specifications

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Glen Research is offering first our most popular labels for general interest and, subsequently, we will add azide products that are not compatible with phosphoramidite chemistry. 7-Hydroxycoumarin, also known as umbelliferone, is a highly fluorescent, pH-sensitive fluorophore that emits in the blue region of the spectrum. However, its fluorescence is strongly quenched if the hydroxyl is alkylated or phosphorylated, making it useful in high-throughput screening for phosphatases and lipases. Interestingly, it was found that the 3-azido derivative is also highly quenched but, upon reaction with an alkyne in the presence of copper to form the triazole, the fluorescence is restored.1 The clicked coumarin emits at a lambda max of 480 nm and absorbs at 358 nm. The azides are offered in 25 and 100 µmole packs for convenient oligonucleotide labelling.


Diluent Not Applicable
Storage Refrigerated storage, maximum of 2-8°C, dry


1 J. Gierlich, G.A. Burley, P.M. Gramlich, D.M. Hammond, and T. Carell, Org Lett, 2006, 8, 3639-42.