Dimethylaminophenothiazin-5-ium chloride, Methylene Blue (MB), has many uses in chemistry and biology. For decades, this compound has been used for applications including redox indicator, photosensitizer, dye for cellular staining procedures, antiseptic and in medicine against Alzheimer’s disease. For the detection of biological analytes, MB has also been used as a redox reporter bound to DNA probes. We offer Methylene Blue NHS Ester to allow simple conjugation to the variety of amino modifiers we supply.
Coupling: For labeling 0.2µmoles of an amino-modified oligonucleotide, dissolve 5.4mg of the Methylene Blue NHS Ester in 60µL of DMSO (Acetonitrile or DMF are also acceptable). Dissolve the amino-modified oligonucleotide in 500µL 0.1 M Carbonate/Bicarbonate buffer (pH 9). Add 12µL of the Methylene Blue NHS Ester solution and vortex. Let react for 2 hours at room temperature and desalt.