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What Are Amino Modifiers?

Labeling or immobilization of oligonucleotides by conjugation reactions requires the presence of functional groups with unique reactivity towards suitably activated dyes, reporter groups or surfaces. Most commonly, amino-modified oligonucleotides are reacted with active esters of various compounds, thereby forming stable amide conjugates. For the introduction of such amino groups at the 5’ terminus of oligonucleotides, 5’ amino-modifiers are employed. They consist of a phosphoramidite moiety and a suitably protected amino group connected by linkers of various length and structure. Depending on the planned purification, amino linkers with acid or base labile amine protecting groups (for DMT-on or DMT-off applications, respectively) can be used. The most popular among the base labile protecting groups is the trifluoroacetyl (TFA) group, which is cleaved by ammonia or methylamine.

5’-amino modifiers

Why Use PDA Amino Modifiers?

Glen Research introduced a set of amino modifiers which are protected by a novel phthalic acid diamide (PDA) protecting group. In contrast to the TFA protected amino modifiers, which are viscous oils, the analogous PDA protected compounds are granular powders. This important property of these compounds allows straightforward handling, storage and aliquoting and leads to a significant increase in stability. For example, at room temperature and under a normal atmosphere, the solid PDA protected amino modifiers are completely stable for at least 7 days. In sharp contrast, the corresponding TFA protected compounds are almost completely degraded under these conditions. Deprotection with methylamine in gas phase or aqueous solution or ammonia-methylamine (AMA) leads to fast and complete removal of the PDA protecting group.

Features and Benefits:

  • Rigorous shipping and storage capability
  • Ease of handling
  • Lower risk of degradation
  • High conjugation efficiency
  • Yields the same product as TFA amino modifiers following deprotection
  • Compatible with different deprotection protocols

Glen Research offers three PDA Amino-Modifiers. Our 5'-Amino-Modifier C6 is our most popular modifier so we offer 5'-Amino-Modifier C6-PDA. For longer linkers, we offer the hydrophobic 5'-Amino-Modifier C12-PDA and the hydrophilic 5'-Amino-Modifier-TEG-PDA.

Deprotection of Oligonucleotides with PDA Amino Modifiers

PDA-off amino-modified oligonucleotide and its biotin conjugate

Figure 1: PDA-off amino-modified oligonucleotide and its biotin conjugate

Deprotection with methylamine in gas phase or aqueous solution or AMA leads to fast and complete removal of the PDA protecting group, as shown in Figure 1. However, ammonium hydroxide will not drive the reaction to completion and only partial deprotection occurs.

High Conjugation Efficiency

PDA-off amino-modified oligonucleotide and its biotin conjugate

Figure 2: PDA-off amino-modified oligonucleotide and its biotin conjugate

The high conjugation efficiency of amino-modified oligonucleotides prepared using PDA 5'-Amino-Modifiers is demonstrated in Figure 2. A T12 oligonucleotide was modified using 5'-Amino-Modifier C12-PDA. Following deprotection using AMA, very high coupling efficiency was observed. Further, the 5'-amino group was shown to have excellent conjugation potential by reaction with biotin NHS Ester in the normal fashion. Essentially quantitative conjugation efficiency was observed (Figure 2).

Product Information

  ProductCatalog NumberPack Sizes
  5'-Amino-Modifier C6-PDA10-1947100 µmol, 0.25 g
  5'-Amino-Modifier C12-PDA10-1948100 µmol, 0.25 g
  5'-Amino-Modifier TEG PDA10-1949100 µmol, 0.25 g