All Glen Research supports use the standard long chain alkylamino (lcaa) linker but differ in the glass pore size, 500Å, 1000Å or 2000Å. The 500Å support is appropriate for shorter sequences, while the 1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences. The 2000Å support is best for very long (>150-mer) oligonucleotides. We have instituted an additional QC test for supports to show the length of oligo that can be prepared before a drop-off in coupling due to steric effects begins to occur. The drop-off point is recorded in the Certificate of Analysis. All Glen Research supports are fully end-capped to ensure that the CPG surface is totally inert, thereby avoiding the introduction of impurity sequences containing deletions at the 3’-terminus.
The 500Å support is appropriate for the synthesis of shorter oligonucleotide sequences
1000Å supports perform better in the synthesis of longer (>30-mer) DNA sequences.
The 2000Å support is best for very long (>150-mer) oligonucleotides.
We are offering Q-linkers of the four regular nucleosides on 500Å CPG in 0.2 and 1µmole scales for quick cleavage.
Our high loading support is based on controlled pore silica and it retains the usual 500Å pores.
Glen Research offers polystyrene supports for the ABI 3900 DNA Synthesizer.
Glen Research offers low-volume supports for oligonucleotide synthesis.