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2'-OMe-A-CE Phosphoramidite - (10-3100)

5'-Dimethoxytrityl-N-benzoyl-Adenosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3100

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Product Specifications

Formula:

C₄₈H₅₄N₇O₈P

M.W.:

887.97

F.W.:

343.24

CAS Number:

110782-31-5

5'-Dimethoxytrityl-N-benzoyl-Adenosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research 2'-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Details

Usage

Coupling: 6 minute coupling time
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	3-5 DAYS

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3100-02	0.25 g	.25grams	2.82	80.67	48.4	30.25	22	16.13	4.03
10-3100-05	0.5 g	.5grams	5.63	174.33	104.6	65.38	47.55	34.87	8.72
10-3100-10	1.0 g	1grams	11.26	362	217.2	135.75	98.73	72.4	18.1
10-3100-90	100 µmol	.089grams	1	20	12	7.5	5.45	4	1

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3100-02	0.25 g	.25grams	4.2	0.07	77.6	48.5	35.27	4.85
10-3100-05	0.5 g	.5grams	8.4	0.07	161.6	101	73.45	10.1
10-3100-10	1.0 g	1grams	16.81	0.07	329.8	206.13	149.91	20.61
10-3100-90	100 µmol	.089grams	1.5	0.07	23.6	14.75	10.73	1.48