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2'-OMe-G-CE Phosphoramidite - (10-3121)

5'-Dimethoxytrityl-N-dimethylaminomethylidene-Guanosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3121

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Product Specifications

Formula:

C₄₄H₅₅N₈O₈P

M.W.:

854.93

F.W.:

359.24

CAS Number:

128219-77-2

5'-Dimethoxytrityl-N-dimethylaminomethylidene-Guanosine,2'-O-methyl,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research 2'-OMe-RNA CE (ß-cyanoethyl) Phosphoramidites are designed to produce synthetic oligonucleotides containing nuclease resistant 2'-O-methyl ribonucleotide linkages. Deprotection, isolation and handling of 2'-O-methyl oligonucleotides are identical to the procedures for oligodeoxynucleotides.

Details

Usage

Coupling: 6 minute coupling time recommended
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	3-5 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3121-02	0.25 g	.25grams	2.92	84	50.4	31.5	22.91	16.8	4.2
10-3121-05	0.5 g	.5grams	5.85	181.67	109	68.13	49.55	36.33	9.08
10-3121-10	1.0 g	1grams	11.7	376.67	226	141.25	102.73	75.33	18.83
10-3121-90	100 µmol	.085grams	1	20	12	7.5	5.45	4	1

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3121-02	0.25 g	.25grams	4.36	0.07	80.8	50.5	36.73	5.05
10-3121-05	0.5 g	.5grams	8.73	0.07	168.2	105.13	76.45	10.51
10-3121-10	1.0 g	1grams	17.46	0.07	342.8	214.25	155.82	21.43
10-3121-90	100 µmol	.085grams	1.5	0.07	23.6	14.75	10.73	1.48