5’-Fluorescein phosphoramidite contains no 4,4’-dimethoxytrityl (DMT) group and can be added only once at the 5’-terminus, thereby terminating synthesis. This product is prepared using the 6-carboxyfluorescein derivative. The tetrachloro-, hexachloro-and dichloro-dimethoxy-fluorescein (TET, HEX and JOE, respectively) phosphoramidites are designed to take advantage of the multicolor detection capability of modern DNA sequencers and genetic analyzers. Fluorescein phosphoramidite is designed to produce the same fluorescein-type structure as had been previously prepared using fluorescein isothiocyanate (FITC). Our fluorescein phosphoramidite also contains a DMT group to allow quantification of coupling. The analogous structure, 6-Fluorescein Phosphoramidite, prepared using 6-FAM, is also available, along with 6-Fluorescein Serinol Phosphoramidite. Fluorescein-dT can be inserted into the desired sequence as a replacement for a dT residue.
The spectral characteristics of this dye is detailed here.
|Storage||Freezer storage, -10 to -30°C, dry|
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
|Catalog #||Pack Size||Grams/Pack||0.1M Dil. (mL)||Approximate Number of Additions|
|Catalog #||Pack Size||Grams/Pack||Dilution (mL)||Approximate Number of Additions|