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Pyrrolo-C-TOM-CE Phosphoramidite - (10-3017)

5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3017

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Product Specifications

Formula:

C₅₂H₇₂N₅O₉PSi

M.W.:

970.23

F.W.:

343.27

CAS Number:

682335-08-6

5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups. Pyrrolo-C is a fluorescent nucleoside whose fluorescence is sensitive to its environment and is ideal for probing RNA structure. It base-pairs as a normal C nucleotide. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-CTP has potential uses in biological assay development.

Details

Usage

Coupling: 6 minute coupling time recommended
Deprotection: Ethanolic Ammonium Hydroxide (1:3) at room temperature for 24 hours or as required by the nucleobases. Remove silyl protecting group according to standard RNA procedures.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3017-02	0.25 g	.25grams	2.58	72.67	43.6	27.25	19.82	14.53	3.63
10-3017-90	100 µmol	.097grams	1	20	12	7.5	5.45	4	1
10-3017-95	50 µmol	.049grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3017-02	0.25 g	.25grams	3.85	0.07	70.6	44.1	14.71	4.4
10-3017-90	100 µmol	.097grams	1.5	0.07	23.6	14.7	4.92	1.4
10-3017-95	50 µmol	.049grams	0.75	0.07	8.6	5.3	1.79	0.5