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Pyrrolo-dC-CE Phosphoramidite - (10-1017)

5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-2'-deoxyribonucleoside, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-1017

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Product Specifications

Formula:

C₄₂H₅₀N₅O₇P

M.W.:

767.85

F.W.:

327.23

CAS Number:

644962-92-5

5'-Dimethoxytrityl-[6-methyl-pyrrolo-[2,3-d]-pyrimidine-2(3H)-one]-2'-deoxyribonucleoside, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Pyrrolo-dC is a fluorescent deoxycytidine analog that is an ideal probe of DNA structure and dynamics. It base-pairs as a normal dC nucleotide. An oligo fully substituted with pyrrolo-dC has the same Tm as the control dC oligo with the same specificity for dG. Its small size does not perturb the structure of the DNA helix and it is well tolerated by a number of DNA and RNA polymerases. It is highly fluorescent and its excitation and emission are well to the red of most fluorescent nucleotide analogs, which eliminates or reduces background fluorescence from proteins. Pyrrolo-dCTP has potential uses in biological assay development.

Details

Usage

Coupling: Use 0.5 M CSO in anhydrous ACN (40-4632-xx) and a 3 minute oxidation time.
Deprotection: Ammonium Hydroxide for 24 hours at room temperature.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-1017-02	0.25 g	.25grams	3.26	95.33	57.2	35.75	26	19.07	4.77
10-1017-90	100 µmol	.077grams	1	20	12	7.5	5.45	4	1
10-1017-95	50 µmol	.038grams	0.5	3.33	2	1.25	0.91	0.67	0.17

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-1017-02	0.25 g	.25grams	4.86	0.07	90.8	56.75	41.27	5.68
10-1017-90	100 µmol	.077grams	1.5	0.07	23.6	14.75	10.73	1.48
10-1017-95	50 µmol	.038grams	0.75	0.07	8.6	5.38	3.91	0.54