2,6-Diaminopurine-TOM-CE Phosphoramidite (2-amino-A) - (10-3085)
5'-Dimethoxytrityl-N6-isobutyryl-N2-methoxyacetyl-2,6-diaminopurine riboside, 2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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10-3085
5'-Dimethoxytrityl-N6-isobutyryl-N2-methoxyacetyl-2,6-diaminopurine riboside, 2'-O-triisopropylsilyloxymethyl-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Description
Glen Research offers minor RNA phosphoramidites with either TOM or TBDMS protecting groups.
The protecting scheme for 2,6-Diaminopurine has been changed and the original product (10-3084) has been replaced with the optimized product (10-3085) below.
Details
Usage
- Coupling: See technical bulletin for details.
- Deprotection: See technical bulletin for details.
Specifications | |
---|---|
Diluent | Anhydrous Acetonitrile/Dichloromethane 1:3 (v/v) |
Recommended Storage | Freezer storage, -10 to -30°C, dry |
Stability In Solution | 2-3 days |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
---|---|---|---|---|---|---|---|---|---|
LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
10-3085-02 | 0.25 g | .25grams | 2.25 | 61.67 | 37 | 23.13 | 16.82 | 12.33 | 3.08 |
10-3085-90 | 100 µmol | .111grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
10-3085-95 | 50 µmol | .056grams | 0.5 | 3.33 | 2 | 1.25 | 0.91 | 0.67 | 0.17 |
Expedite
Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
---|---|---|---|---|---|---|---|---|
Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
10-3085-02 | 0.25 g | .25grams | 3.35 | 0.07 | 60.6 | 37.8 | 12.63 | 3.7 |
10-3085-90 | 100 µmol | .111grams | 1.5 | 0.07 | 23.6 | 14.7 | 4.92 | 1.4 |
10-3085-95 | 50 µmol | .056grams | 0.75 | 0.07 | 8.6 | 5.3 | 1.79 | 0.5 |
Technical documents
Certificate of analysis
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