Glen Report 24.11: Spermine Phosphoramidite: A Potent Modification with Many Applications

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67400 Illkirch, France

Introduction to ZNA® Oligos

Synthetically derived oligonucleotides have found many uses in molecular biology, diagnostic and therapeutic applications. At the heart of the majority of these applications is the canonical Watson-Crick base-pairing of nucleobases, whereby complementary strands form duplexes with each other. However, the strength of such interactions is tempered by the mutual electrostatic repulsion of the negatively charged phosphate backbones running through each strand. This polyanionic nature of oligonucleotides also hinders their ability to enter cells and access therapeutically interesting targets that are in the cell cytosol or nucleus.

Figure 1
Oligonucleotide Oligospermine Conjugate

Chemists have long sought to address the issue of electrostatic repulsion to improve both the binding of an oligonucleotide to a target sequence and its cell delivery. Several approaches aimed at reducing the anionic charge can be found in the literature ranging from the replacement of the phosphate backbone with one that is amide based, such as in Peptide Nucleic Acids (PNA),1 or the introduction of ammonium or guanidinium residues throughout the strand,2,3 to the conjugation of polycations that preserve the hybridization specificity and nucleic acid-processing enzyme activity of the parent oligonucleotide.4 In the case of the latter, a convenient way of introducing a polycation polymer is by the use of the spermine phosphoramidite (Figure 1), which can be coupled in an iterative manner similar to standard phosphoramidites.5,6 The resulting oligonucleotide-oligospermine conjugates (illustrated above and in Figure 2) are known as “Zip Nucleic Acids” or “ZNA®”,7 a term that reflects the presumed mode of action of the conjugates that are believed to use the oligospermine to seek out and move along (scan) oligonucleotide strands until the probe complementary sequence is located. The oligospermine then performs the function of stabilizing the formed duplex by reducing electrostatic repulsion, thereby leading to significantly increased binding affinities. Despite this increase, ZNA® oligonucleotides still retain the ability to discriminate mismatches, doing so to the same extent as unmodified DNA.8 Moreover, the oligospermine can be located at the 3’ or 5’ terminus depending on the application, producing the same beneficial effects to the binding affinity.

Applications of ZNA® Oligos

Since their inception, an increasing number of applications are being found for ZNA® that take advantage of their unique properties. For example, their use as primers for PCR and reverse transcription has been examined.9 ZNA® oligos were found to drive PCR reactions very efficiently at higher annealing temperatures, substantially lower primer, substrate and magnesium concentrations, and faster cycling timeframes compared to DNA and LNA primer controls. The latter feature is a result of the “Zip” character of ZNA®, which also results in higher probe sensitivity presumably by increasing the local concentration of the probe in solution. The primer Tm could be easily and predictably tuned simply by the addition of spermine units. Such properties make ZNA® ideal for multiplex PCR and PCR of AT-rich regions that are typically problematic due to the low Tm of the corresponding duplexes. Figure 3 shows the comparative PCR performance of ZNA® and DNA primers on an AT-rich sequence.

Figure 1: Structure of spermine phosphoramidite
(1) Spermine phosphoramidite
Figure 2: Oligonucleotide oligospermine conjugate

Depiction of ZNA® molecule whereby the oligospermine tail is attached to the 5’ of a DNA oligomer. The net charge is determined by the formula 3n-(m-1), where m is the oligonucleotide length and n the number of spermine units.

ZNA® oligos also have notable advantages when used as primers for reverse transcription (RT).9 In this respect, it was shown that ZNA® primers improve the yield of RNA to cDNA conversion under standard conditions that led to earlier detection in RT-qPCR quantifications compared to DNA. As a result, this leads to more accurate quantification of low-abundant transcripts. Moreover, the primers in such applications were found to be more robust to variations in buffer environment compared to unmodified DNA primers.

Figure 4
Figure 3: Comparative PCR performance of ZNA®and DNA primers on an AT-rich Sequence

ZNA® primers (Red) used for AT-rich regions in comparison to the corresponding LNA and DNA primers (Black) at 100nM primer concentration and varying copies of initial template following a standard 2 step protocol (95°C, 10s followed by 60°C, 30s)

The properties of ZNA® molecules make them ideal for use as dual-labelled hydrolysis probes for qPCR. 3’-attached oligospermine-oligonucleotide conjugates can be made shorter than their non-modified counterparts leading to improved single-nucleotide polymorphism discrimination (Figure 4).8 In addition, longer ZNA® of a length similar to that used for non-modified probes, exhibited reduced background fluorescence through increased quenching, thus generating improved signal-to-noise ratios. Such a finding opens the way for ZNA® to be used as non-hydrolysis probes. Due to the predictable increases in Tm afforded to the molecules by the attached spermine units, it is easier to design ZNA® probes compared to alternative oligonucleotide modifications such as minor groove binders (MGB) or LNA probes. Moreover, it was found that ZNA® tolerated a wider range of conditions than their DNA and LNA counterparts. In a recent report by Trevisan and coworkers,10 ZNA® probes were successfully used for the in-situ detection of microRNAs in plants. The probes were shown to be highly sensitive and selective when compared to LNA probes and indicate a potential for exploitation of ZNA® for high-throughput miRNA profiling applications.

Figure 4
Figure 4: Improved single-nucleotide polymorphism (SNP) discrimination with short ZNA® probes
A 17mer DNA conjugated with 4 spermines (Red) shows better mismatch discrimination compared to an unmodified DNA 22mer (Black).

ZNA® Oligos as antisense and antigene agents

As the efficiency of cell-uptake of oligonucleotides is improved by their conjugation to cationically charged moieties,11 it follows that polyspermine modification also renders ZNA® more amenable to cell delivery. This point has been exploited, for example, by the group of David Corey who assessed the use of ZNA® as antisense and antigene agents.12

In-vitro assays revealed that oligospermine modification of control DNA and LNA/DNA mixmers had a significant beneficial impact on antisense activity when delivered with cationic lipid. For instance, a previously nonfunctional DNA molecule attained antisense activity through the addition of 6 spermine units. More interestingly, free delivery (without cationic lipid mediated transfection) yielded IC50 values in the mid-nanomolar range (Table 1).

Table 1: Antisense Activity of ZNA® oligonucleotides
Oligonucleotide IC50 (nM) with cationic lipid IC50 (nM) without cationic lipid
DNA >100 >400
Z3-DNA >100 >400
Z6-DNA 30.8 194
Z9-DNA 81.9 119
LNA(T) 30.5 >400
Z3-LNA(T) 16.4 >400
Z6-LNA(T) 30.2 >400
Z9-LNA(T) 19.1 160
Antisense Activity of ZNA® oligonucleotides in the presence and absence of cationic lipid.12 Spermine (Z) additions to DNA (gctgctgctgctgctgctg) directed towards the CAG repeat of mutant huntingtin (HTT) mRNA triggered antisense activity with and without cationic lipid mediated transfection. The same sequence with LNA substitutions for Thymidine, LNA(T), also showed significant improvement in assisted and free delivery.

A similar outcome was seen with ZNA® oligos used for the antigene application. Oligospermine-ribonucleotide conjugates have also been reported as a way to transport siRNA into cells. Nothisen and coworkers13 synthesized several 5’-oligospermine-sense strand conjugates and determined that an siRNA net cationic charge was necessary to effect efficient cell uptake and gene silencing in the submicromolar range. These encouraging results were also accompanied by an indirect confirmation of the siRNA mechanism, namely that switching the oligospermine to the 5’- of the antisense strand resulted in loss of activity.

ZNA® in Oligo Synthesis

Due to their versatility, compatibility with a variety of other modifications and the ability to fine tune their properties, there is a growing interest in ZNA®. Consequently, Glen Research has now added the spermine phosphoramidite to their catalog.

The spermine amidite can be efficiently incorporated using a 3 minute coupling time, and can be deprotected with standard deprotection conditions in ammonium hydroxide, however some loss of the spermine modification has been observed with extended heating. To avoid acrylonitrile addition to the spermine units, it is necessary to first treat the CPG with 10% DEA in acetonitrile prior to cleavage from the support. Five minutes at room temperature was found to be sufficient time.

Due to the modified properties of the ZNA® molecules, standard reversed-phase purification conditions, i.e., 0.1 M TEAA/acetonitrile, tend to produce broad, smeared peaks. However, it is possible to purify the crude oligos using Glen-Pak™ cartridges (User Guide to Glen-PakTM Purification) as well as RP-HPLC using a Waters X-Bridge column in combination with a high pH buffer system (5 mM TEA in 25 mM TEAA/acetonitrile). High pH is also necessary to purify ZNA® with anion exchange.5,6 In this regard, addition of 10% acetonitrile and warming the column to 35°C can both have beneficial effects on resolution.

With an increase in spermine content, the solubility of ZNA® oligonucleotides may be noticeably less than unmodified DNA or RNA counterparts. This is typically observed when re-dissolving dried-down purified ZNA® in water. In this case, dropwise addition of 50 mM ammonium hydroxide brings ZNA® molecules into solution. Alternatively, dissolving ZNA® oligos in concentrated phosphate buffered saline (2.5x PBS, pH 7.4) has also been found to resolve solubility issues.12

Intellectual Property

"Spermine phosphoramidite" synthon is the subject matter of U.S. Patent Application No. 12/086.599, European Patent Application No. EP20060847298 and foreign equivalents for which Polyplus-transfection is the co-owner. Product is sold for research purposes only. Product shall not be used to manufacture oligonucleotide-oligospermine conjugates for use in diagnostics, clinical or commercial applications including use in humans. There is no implied license to manufacture oligospermine-oligonucleotide conjugates for diagnostic, clinical or commercial applications, including but not limited to contract research.

Please contact Polyplus-transfection at [email protected] to obtain a license for such use.


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  13. Nothisen, M. et al. J. Am. Chem. Soc. 2009, 131, p.17730-17731.

Product Information

Spermine Phosphoramidite (10-1939)