dT-5'-CE Phosphoramidite - (10-0301)
3'-Dimethoxytrityl-2'-deoxyThymidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
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10-0301
3'-Dimethoxytrityl-2'-deoxyThymidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
Description
Glen Research 5'-CE (ß-cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for structural studies.
Details
Usage
- Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
- Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.
| Specifications | |
|---|---|
| Diluent | Anhydrous Acetonitrile |
| Recommended Storage | Refrigerated storage, maximum of 2-8°C, dry |
| Stability In Solution | Similar to dA,C,G,T-CE Phosphoramidites |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 10-0301-02 | 0.25 g | .25grams | 3.36 | 98.67 | 59.2 | 37 | 26.91 | 19.73 | 4.93 |
| 10-0301-05 | 0.5 g | .5grams | 6.71 | 210.33 | 126.2 | 78.88 | 57.36 | 42.07 | 10.52 |
| 10-0301-10 | 1.0 g | 1grams | 13.43 | 434.33 | 260.6 | 162.88 | 118.45 | 86.87 | 21.72 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 10-0301-02 | 0.25 g | .25grams | 5.01 | 0.07 | 93.8 | 58.63 | 42.64 | 5.86 |
| 10-0301-05 | 0.5 g | .5grams | 10.02 | 0.07 | 194 | 121.25 | 88.18 | 12.13 |
| 10-0301-10 | 1.0 g | 1grams | 20.04 | 0.07 | 394.4 | 246.5 | 179.27 | 24.65 |
Technical documents
Certificate of analysis
CoA search tool
Related products
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dmf-dG-5'-CE Phosphoramidite
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dC-5'-CPG
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dG-5'-CPG
Cat. No.: 20-0202
References
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