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dC-5'-CPG - (20-0102)

3'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine, 5'-succinoyl-long chain alkylamino-CPG

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20-0102

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Product Specifications

F.W.:

289.18

Pore Size:

1000Å

3'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine, 5'-succinoyl-long chain alkylamino-CPG

Description

The following supports are used to produce oligonucleotides with nuclease resistant 3’-3’ linkages at the 3’ terminus (by attaching regular 3’-CE phosphoramidites) or to produce oligonucleotide sections in the opposite sense (by attaching 5’-CE phosphoramidites). ABI-style columns are supplied unless otherwise requested (see note box).

Details

Usage

Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
Deprotection: Deprotect using the protocol required by the nucleobases. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in about 5% N4-methyl-dC side product at the modification site.

Specifications
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
20-0102-01	0.1 g	.1grams	0	0	0	0	0	0	0
20-0102-10	1.0 g	1grams	0	0	0	0	0	0	0
20-0112-13	1 x 10 µmol	.272grams	0	0	0	0	0	0	0
20-0112-14	1 x 15 µmol	.408grams	0	0	0	0	0	0	0
20-0112-42	4 x 0.2 µmol	.022grams	0	0	0	0	0	0	0
20-0112-41	4 x 1.0 µmol	.109grams	0	0	0	0	0	0	0

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
20-0102-01	0.1 g	.1grams	0	0	0	0	0	0
20-0102-10	1.0 g	1grams	0	0	0	0	0	0
20-0112-13	1 x 10 µmol	.272grams	0	0	0	0	0	0
20-0112-14	1 x 15 µmol	.408grams	0	0	0	0	0	0
20-0112-42	4 x 0.2 µmol	.022grams	0	0	0	0	0	0
20-0112-41	4 x 1.0 µmol	.109grams	0	0	0	0	0	0