This product has been discontinued.
We have discontinued this product permanently. Please find recommended products below.
Recommended Products
dC-5'-CPG
Catalog #: 20-0102
dT-5'-CPG
Catalog #: 20-0302
dmf-dG-5'-CE Phosphoramidite
Catalog #: 10-9201
dG-5'-CPG - (20-0202)
3'-Dimethoxytrityl-N-isobutyryl-2'-deoxyGuanosine,5'-succinoyl-long chain alkylamino-CPG3'-Dimethoxytrityl-N-isobutyryl-2'-deoxyGuanosine,5'-succinoyl-long chain alkylamino-CPG
Description
The following supports are used to produce oligonucleotides with nuclease resistant 3’-3’ linkages at the 3’ terminus (by attaching regular 3’-CE phosphoramidites) or to produce oligonucleotide sections in the opposite sense (by attaching 5’-CE phosphoramidites). ABI-style columns are supplied unless otherwise requested (see note box).
Details
Usage
- Coupling: This support should be used in a manner identical to normal protected nucleoside support since it contains the DMT group.
- Deprotection: Deprotect using the protocol required by the nucleobases.
| Specifications | |
|---|---|
| Recommended Storage | Controlled room temperature or lower, dry |
Dilution/Coupling Data
The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.
ABI 392/394
| Catalog # | Pack Size | Grams/Pack | 0.1M Dil. (mL) | Approximate Number of Additions | |||||
|---|---|---|---|---|---|---|---|---|---|
| LV40 | LV200 | 40nm | 0.2μm | 1μm | 10μm | ||||
| 20-0202-01 | 0.1 g | .1grams | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Expedite
| Catalog # | Pack Size | Grams/Pack | Dilution (mL) | Approximate Number of Additions | ||||
|---|---|---|---|---|---|---|---|---|
| Molarity | 50nm | 0.2μm | 1μm | 15μm | ||||
| 20-0202-01 | 0.1 g | .1grams | 1 | 1 | 1 | 1 | 1 | 1 |
Technical documents
Related products
Related Products
dC-5'-CPG
Cat. No.: 20-0102
dT-5'-CPG
Cat. No.: 20-0302
dmf-dG-5'-CE Phosphoramidite
Cat. No.: 10-9201
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