Home Products Modification and Labeling Products for Oligonucleotide Backbone Modification 5' -> 3' Synthesis Phosphoramidites and Supports 5'-3' Synthesis Phosphoramidites dmf-dG-5'-CE Phosphoramidite

dmf-dG-5'-CE Phosphoramidite - (10-9201)

3'-Dimethoxytrityl-N-dimethylformamidine-2'-deoxyGuanosine, 5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-9201

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Product Specifications

Formula:

C₄₃H₅₃N₈O₇P

M.W.:

824.92

F.W.:

329.21

CAS Number:

261728-23-8

3'-Dimethoxytrityl-N-dimethylformamidine-2'-deoxyGuanosine, 5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research 5'-CE (ß-cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for structural studies.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	2-3 days

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-9201-02	0.25 g	.25grams	3.03	87.67	52.6	32.88	23.91	17.53	4.38
10-9201-05	0.5 g	.5grams	6.06	188.67	113.2	70.75	51.45	37.73	9.43
10-9201-10	1.0 g	1grams	12.12	390.67	234.4	146.5	106.55	78.13	19.53

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-9201-02	0.25 g	.25grams	4.52	0.07	84	52.5	38.18	5.25
10-9201-05	0.5 g	.5grams	9.05	0.07	174.6	109.13	79.36	10.91
10-9201-10	1.0 g	1grams	18.09	0.07	355.4	222.13	161.55	22.21