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dC-5'-CE Phosphoramidite - (10-0101)

3'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-0101

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Product Specifications

Formula:

C₄₆H₅₂N₅O₈P

M.W.:

833.93

F.W.:

289.18

CAS Number:

140712-83-0

3'-Dimethoxytrityl-N-benzoyl-2'-deoxyCytidine,5'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research 5'-CE (ß-cyanoethyl) Phosphoramidites are designed for the production of 5'-5' or 3'-3' linkages, useful in antisense studies, or to synthesize oligonucleotide segments in the opposite sense from normal synthesis, for structural studies.

Details

Usage

Coupling: No changes needed from standard method recommended by synthesizer manufacturer.
Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. Note: Deprotection in AMA (50:50 Ammonium hydroxide/MethylAmine) may result in about 5% N4-methyl-dC side product at the modification site.

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Refrigerated storage, maximum of 2-8°C, dry
Stability In Solution	Similar to dA,C,G,T-CE Phosphoramidites

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-0101-02	0.25 g	.25grams	3.05	86.67	52	32.5	23.64	17.33	4.33
10-0101-05	0.5 g	.5grams	6	186.67	112	70	50.91	37.33	9.33
10-0101-10	1.0 g	1grams	11.99	386.33	231.8	144.88	105.36	77.27	19.32

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-0101-02	0.25 g	.25grams	4.47	0.07	83.55	51.88	37.73	5.19
10-0101-05	0.5 g	.5grams	8.95	0.07	172.6	107.88	78.45	10.79
10-0101-10	1.0 g	1grams	17.9	0.07	351.6	219.75	159.82	21.98