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Ac-C-CE Phosphoramidite - (10-3015)

5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

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10-3015

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Product Specifications

Formula:

C₄₇H₆₄N₅O₉PSi

M.W.:

902.11

F.W.:

305.18

CAS Number:

121058-88-6

5'-Dimethoxytrityl-N-acetyl-Cytidine,2'-O-TBDMS-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Description

Glen Research CE (ß-cyanoethyl) Phosphoramidites for RNA synthesis are produced and packaged to ensure the highest performance on commercial synthesizers. Every batch is accompanied by a Certificate of Analysis and an HPLC trace, showing the results of our QC testing. RNA Phosphoramidites are synthesis-tested with a minimum coupling efficiency of 97%. Glen Research RNA monomers are packaged in industry standard vials which are specially cleaned to eliminate particulate contamination. These monomers are available in a variety of packs, including high throughput (HT) and low cost (LC). An UltraMild set is also available for situations where sensitive bases are in use. Dmf-G (10-3029) has been discontinued and may be substituted with Ac-G (10-3025).

Details

Usage

Coupling: 12 minute coupling time recommended
Deprotection: RNA Deprotection-see bulletin

Specifications
Diluent	Anhydrous Acetonitrile
Recommended Storage	Freezer storage, -10 to -30°C, dry
Stability In Solution	24 hours

Dilution/Coupling Data

The table below show pack size data and, for solutions, dilution and approximate coupling based on normal priming procedures.

ABI 392/394

Catalog #	Pack Size	Grams/Pack	0.1M Dil. (mL)	Approximate Number of Additions
				LV40	LV200	40nm	0.2μm	1μm	10μm
10-3015-02	0.25 g	.25grams	2.77	79	47.4	29.63	21.55	15.8	3.95
10-3015-05	0.5 g	.5grams	5.54	171.33	102.8	64.25	46.73	34.27	8.57
10-3015-10	1.0 g	1grams	11.09	356.33	213.8	133.63	97.18	71.27	17.82

Expedite

Catalog #	Pack Size	Grams/Pack	Dilution (mL)	Approximate Number of Additions
				Molarity	50nm	0.2μm	1μm	15μm
10-3015-02	0.25 g	.25grams	4.14	0.07	76.4	47.7	15.92	4.7
10-3015-05	0.5 g	.5grams	8.27	0.07	159	99.3	33.13	9.9
10-3015-10	1.0 g	1grams	16.54	0.07	324	202	67.58	20.2